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brij 35  (Thermo Fisher)
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Thermo Fisher brij 35
Brij 35, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brij 35  (Alpha Chemika)
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w v brij 35 anatrace  (Anatrace)
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A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
W V Brij 35 Anatrace, supplied by Anatrace, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brij 35  (Loba Chemie)
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Loba Chemie brij 35
A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
Brij 35, supplied by Loba Chemie, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary antibody  (Thermo Fisher)
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Thermo Fisher secondary antibody
A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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secondary antibody - by Bioz Stars, 2026-09
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resource source identifier antibodies goat anti mouse igg invitrogen  (Thermo Fisher)
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Thermo Fisher resource source identifier antibodies goat anti mouse igg invitrogen
A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
Resource Source Identifier Antibodies Goat Anti Mouse Igg Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase  (Thermo Fisher)
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A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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horseradish peroxidase hrp  (Thermo Fisher)
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Thermo Fisher horseradish peroxidase hrp
A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
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scientific cat 31460 rrid ab 228341  (Thermo Fisher)
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Thermo Fisher scientific cat 31460 rrid ab 228341
A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), <t>Brij-35,</t> 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.
Scientific Cat 31460 Rrid Ab 228341, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), Brij-35, 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.

Journal: bioRxiv

Article Title: A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM

doi: 10.64898/2026.03.17.712260

Figure Lengend Snippet: A ) Sankey plot showing documented surfactant additives for preferred orientation (PO) in publications referenced in the EMDB. Nodes separate publications using surfactant(s) to overcome preferred orientation ( left ), surfactant class by chemical properties ( middle ), and surfactant identity ( right ). B ) Violin plot showing concentrations of all single-additive use cases relative to the critical micelle concentration (CMC) for each respective surfactant: octyl glucoside (OG), nonidet P-40 (NP40), lauryl maltose neopentyl glycol (LMNG), fluorinated octyl maltoside (FOM), Brij-35, 3-[(3-cholamidopropyl)-dimethylammonio]-2-hydroxy-1-propanesulfonate (CHAPSO), 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate (CHAPS), and fluorinated fos-choline-8 (FFC-8). Amphipol 8-35 (A8-35) was excluded from this analysis since it does not possess a traditional CMC.

Article Snippet: A 20× SurfACT solution was prepared of 1% (w/v) 3-([3-cholamidopropyl]-dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO; Anatrace), 0.096% (w/v) 1H, 1H, 2H, 2H-perfluorooctyl)- β -D-maltopyranoside (FOM; Anatrace), 5% (w/v) amphipol A8-35 (Anatrace), and 1.2% (w/v) Brij-35 (Anatrace), stored in aliquots at −20°C.

Techniques: Concentration Assay